En
  • دکتری (1386)

    هماتولوژی بالینی

    یاماگوچی، ژاپن

  • کارشناسی‌ارشد (1377)

    خون شناسی

    دانشگاه تربیت مدرس،

  • کارشناسی (1373)

    زیست شناسی

    دانشگاه های پیام نور،

  • بیولوژی و پاتوبیولوژی و درمان مالتیپل میلوما
  • تمایز و تکثیر سلولهای بنیادی و کاربرد بالینی
  • سرطان
  • مباحث مولکولی (سیگنالهای درون سلولی و تنظیم بیان ژن و پروتئین)
  • گیاهان دارویی
  • مقاومت به شیمی درمانی
  • استفاده از سیستم CRISPER در اصلاح ژن تالاسمی

    داده ای یافت نشد

    The Inhibition of Proliferation and Invasion of Human Colon Carcinoma Cell Line (Caco-2 cells) by Cell-free Supernatants from Lactobacillus Rhamnosus and Lactobacillus Acidophilus

    Ameneh Shokati, Masoud Soleimani, Saeid Abroun
    Journal Papers , 2021 January 27, {Pages }

    Abstract

    Background: The epidemiological studies indicated that colorectal cancer is one of the most common types of cancer in the world and is considered a leading cause of cancer-related death. The present study aimed to investigate the inhibitory effect of lactobacillus acidophilus supernatant (LAS) and lactobacillus rhamnosus supernatant (LRS) on the growth and invasiveness of the human colon carcinoma cell line (Caco2) in-vitro.Methods: In this experimental study, the anti-proliferative activity and anti-invasion potential of LAS and LRS were determined by MTT and transwell chambers assays, respectively. The expression of mitochondrial membrane potential-9 (MMP-9) and matrix metalloproteinase-12 (MMP12) genes were analyzed by real-time PCR.Resu

    Mesenchymal stem cell‐derived extracellular vesicles conditionally ameliorate bone marrow failure symptoms in an immune‐mediated aplastic anemia mouse model

    Mohammad A Gholampour, Saeid Abroun, Rienk Nieuwland, Seyed J Mowla, Sara Soudi
    Journal PapersJournal of cellular physiology , 2021 January 25, {Pages }

    Abstract

    Acquired forms of Aplastic anemia (AA) are characterized by T cell‐mediated immune disease resulting in bone marrow (BM) failure and marrow hypoplasia. In these cases, it is a major challenge to modulate autoreactive T cell activity and thereby decrease the pro‐inflammatory cytokine storm. Emerging evidence indicates that extracellular vesicles derived from mesenchymal stem cells (MSC‐EVs) control and modulate immunity. The therapeutic potential of MSC‐EVs has not been investigated in acquired AA. Hence, in this study, we constructed an AA mice model through irradiation and splenocyte infusion to test the benefits of hypoxic MSC‐EVs (Hx‐EVs) and normoxic MSC‐EVs (Nx‐EVs). We found that MSC‐EVs treatment significantly prol

    Breast Cancer Cell Lines, HER2/Neu Phenotype, and a Higher Propensity to Reactive Oxygen Species Production

    Zahra Mohammadi Abgarmi, Abbas Sahebghadam Lotfi, Saeid Abroun, Masoud Soleimani, Shahla Mohammad Ganji, Parvaneh Baktash, Arash Moradi
    Journal PapersArchives of Breast Cancer , 2021 April 15, {Pages 137-142 }

    Abstract

    Background: The reactive oxygen species (ROS) generated in the biological systems play an important role in pathological conditions and specific normal cellular processes, such as signaling pathways and drug sensitivity.Methods: Intracellular ROS was determined using (2'-7'dichlorofluorescin diacetate) DCFH-DA fluorimetric probe, malondialdehyde as lipid peroxidation index detected by the Thiobarbituric acid reactive substances method. Cells' protein carbonyl contents were assessed with 2, 4-Dinitrophenylhydrazine (DNPH) derivatization spectrophotometrically at 360-385 nm.Results: The oxidative stress induces ROS (p? 0.05), MDA (Malondialdehyde)(p? 0.05), and protein carbonylation (p? 0.01) was significantly higher in HER2-positive BT-474,

    AntagomiR-19a Induced Better Responsiveness to Bortezomib in Myeloma Cell Lines

    A Kazemi, S Abroun, M Soleimani
    Journal Papers , , {Pages }

    Abstract

    Stem Cell Therapy: A Promising Approach in Treatment of COVID-19

    B Abbaspanah, S Abroun, M Zarrabi, A Mozdgir, M Mollanouri
    Journal Papers , , {Pages }

    Abstract

    Indirect Tumor Inhibitory Effects of MicroRNA-124 through Targeting EZH2 in The Multiple Myeloma Cell Line

    Javid Sabour Takanlu, Arad Aghaie Fard, Saeed Mohammdi, Seyed Mohammad Ali Hosseini Rad, Saeid Abroun, Mohsen Nikbakht
    Journal PapersCell Journal (Yakhteh) , Volume 22 , Issue 1, 2020 March 1, {Pages }

    Abstract

    Objective: Multiple myeloma (MM) is an incurable plasma cell malignancy. Several genetic and epigenetic changes affect numerous critical genes expression status in this disorder. CDKN2A gene is expressed at low level in almost all cases with MM disease. The mechanism of this gene down-regulation has remained controversial. In the present study, we targeted EZH2 by microRNA-124 (miR-124) in L-363 cells and assessed following possible impact on CDKN2A gene expression and phenotypic changes.Materials and Methods: In this experimental study, growth inhibitory effects of miR-124 were measured by MTT assay in L-363 cell line. Likewise, cell cycle assay was measured by flowcytometery. The expression levels of EZH2 and CDKN2A were evaluated by real

    Evaluation of stemness genes expression of OCT4, SOX2, Nanog, C-Myc and surface marker of CD133 on myeloma cells

    Saeid Abroun, Masoud Soleimani
    Journal PapersJournal of Research in Applied and Basic Medical Sciences , Volume 6 , Issue 2, 2020 April 10, {Pages 96-103 }

    Abstract

    Cancer stem cells that have the ability to proliferation and self-renewal were cause to drug resistance and metastasis in malignancies. In multiple myeloma, these cancer stem cells (MMCSCs) play a very important role in the recurrence of the disease. Detection of these cells can create new ways for treating these patients. In this study, stemness genes and phenotypic markers were investigated as the methods for identifying cancer stem cells in multiple myeloma. Expression of stemness genes of OCT4, SOX2, Nanog, C-Myc and target genes of STAT3 and TCF3 by PCR and Real Time PCR and also expression of surface markers of CD19, CD33, CD133, CD38 and CD56 by flow cytometry in RPMI8226 and U266 were studied. The results of the study showed that my

    Factor V Leiden 1691G> A mutation and the risk of recurrent pregnancy loss (RPL): systematic review and meta-analysis

    Mohammad Masoud Eslami, Mina Soufizomorrod, Saeid Abroun, Bahman Razi
    Journal Papers , Volume 18 , Issue 1, 2020 December , {Pages 16-Jan }

    Abstract

    Although numerous replication case-control studies have attempted to determine the association between Factor V Leiden (FVL) 1691G > A mutation and susceptibility to Recurrent pregnancy loss (RPL), there have been confliction among the results of various ethnic groups. To address this limitation, here we implemented first meta-analysis to provide with consistent conclusion of the association between FVL 1691G > A mutation and RPL risk. After a systematic literature search, pooled odds ratio (OR) and their corresponding 95% confidence interval (CI) were used to evaluate the strength of the association. Additionally, meta-regression analyses were performed to find potential source of heterogeneity. In this meta-analysis, 62 studies, c

    Induced Pluripotent Stem Cell Meets Severe Combined Immunodeficiency

    Reza Kouchaki, Bahareh Abd-Nikfarjam, Amirhosein Maali, Saeid Abroun, Farshad Foroughi, Sasan Ghaffari, Mehdi Azad
    Journal Papers , Volume 22 , Issue Suppl 1, 2020 January , {Pages 1 }

    Abstract

    Severe combined immunodeficiency (SCID) is classified as a primary immunodeficiency, which is characterized by impaired T-lymphocytes differentiation. IL2RG, IL7Ralpha, JAK3, ADA, RAG1/RAG2, and DCLE1C (Artemis) are the most defective genes in SCID. The most recent SCID therapies are based on gene therapy (GT) of hematopoietic stem cells (HSC), which are faced with many challenges. The new studies in the field of stem cells have made great progress in overcoming the challenges ahead. In 2006, Yamanaka et al. achieved" reprogramming" technology by introducing four transcription factors known as Yamanaka factors, which generate induced pluripotent stem cells (iPSC) from somatic cells. It is possible to apply iPSC-derived HSC for transplantati

    Plasma Cell Proliferation Is Reduced in Myeloma-Induced Hypercalcemia and in Co-Culture with Normal Healthy BM-MSCs

    Nader Vazifeh Shiran, Saeid Abroun
    Journal PapersLaboratory Medicine , 2020 October 14, {Pages }

    Abstract

    Objective In multiple myeloma (MM), stimulation of osteoclasts and bone marrow (BM) lesions lead to hypercalcemia, renal failure, and anemia. Co-culture of the myeloma cells in both hypocalcemia and hypercalcemia concentrations with bone marrow-mesenchymal stem cells were evaluated. Materials and Methods Viability and survival of myeloma cells were assessed by microculture tetrazolium test and flow cytometric assays. Mesenchymal stem cells (MSCs) were extracted from normal and myeloma patients and were co-cultured with myeloma cells. Results Myeloma cells showed less survival in both hypocalcaemia and hypercalcemia conditions (P <.01)

    Stem Cell Therapy: A Promising Approach in Treatment of COVID 19.

    Bahareh Abbaspanah, Saeid Abroun, Morteza Zarrabi, Ashkan Mozdgir, Mohammad Mollanouri
    Journal Papers , 2020 October 12, {Pages }

    Abstract

    COVID-19 pandemic is a global health crisis in the 21st Century. There are currently no approved vaccine and no particular antiviral treatment for coronavirus disease. As COVID-19 has had a broad range of illnesses, it is necessary to find a safe and effective therapeutic method for COVID-19. An attractive approach for treating COVID-19 is cell therapy. Cell therapy aims to inject new and healthy stem cells into a patient's body, to repair the damaged cells and tissues. Stem cell therapy is one of the most studied and important approaches in treatment of COVID-19 these days. The significant clinical outcome was observed by the adoptive transfer of stem cells, specifically mesenchymal stem cells. This study reviews the characteristics of ste

    Inhibition of miR-19a-3p Increases Bortezomib-Induced Apoptosis in Myeloma Cell Lines by Targeting SOCS3

    Azam KAZEMI, Saeid ABROUN, Masoud SOLEIMANI
    Journal PapersTURKISH JOURNAL OF ONCOLOGY , Volume 1 , Issue 3, 2020 January , {Pages }

    Abstract

    OBJECTIVEApoptosis is considered the main factor inducing by bortezomib in myeloma cells. Although bortezomib (BTZ) is a crucial drug for the treatment of MM, chemoresistance is a major problem. OncomiR-19a plays an oncogenic role in many types of cancer such as MM; however, the function of miR-19a in the pathogenesis of MM and drug resistance has not been identified well. The present research aims at investigating the inhibition of miR-19a by antagomir to conclude BTZ responsiveness and suggests that miR-19a may be a biomarker for the prognosis of the patient.

    The Expression of Microvesicles in Leukemia: Prognostic Approaches.

    Ali Ehsanpour, Najmaldin Saki, Marziye Bagheri, Masumeh Maleki Behzad, Saeid Abroun
    Journal PapersCell Journal (Yakhteh) , Volume 12 , Issue 2, 2019 July 1, {Pages }

    Abstract

    Microvesicles (MVs) are the smallest subclass of the extracellular vesicles (EVs) spontaneously secreted by the external budding from the cell membranes in physiologic and pathologic conditions. The MVs derived from leukemic cells (LCs) can be detected by the expression of specific cluster of differentiation (CD) markers indicating their cellular origin while they can transfer different agents such as microRNAs, cytokines, and chemokines. The secretion of these agents from MVs can affect the vital processes of LCs such as cell cycle, proliferation, differentiation, and apoptosis. According to the effects of MVs components on the vital processes of LCs, it has been postulated that a change in the expression of MVs might be involved in the pr

    Comparison of cord blood CD34+ stem cell expansion in coculture with mesenchymal stem cells overexpressing SDF‐1 and soluble/membrane isoforms of SCF

    Mansoureh Ajami, Masoud Soleimani, Saeid Abroun, Amir Atashi
    Journal PapersJournal of cellular biochemistry , 2019 May 17, {Pages }

    Abstract

    Aim Umbilical cord blood hematopoietic stem cells (UCB HSCs) have been considered for the treatment of hematological malignancies due to their noninvasive collection, greater capacity of expansion, and remarkable tolerance for HLA mismatch in transplantation. On the other hand, the most considerable limitation of these cells is their inadequate amount of CD34 + HSCs which leads to delayed engraftment. The aim of this study was the expansion of CD34 + HSCs by coculturing with mesenchymal stem cells (MSCs) overexpressing stromal cell‐derived factor‐1, soluble and membrane isoforms of stem cell factor (sSCF/mSCF). Keeping structure and function of overexpressed cytokines by MSCs was expected which could beneficially affect the CD

    Amniotic fluid, an effective factor for umbilical cord blood hematopoietic stem cells in cell culture: An approach for bone marrow transplantation

    Tayebe Torabi, Saeid Abroun
    Journal PapersTransfusion and Apheresis Science , Volume 58 , Issue 2, 2019 April 1, {Pages 169-173 }

    Abstract

    Bone marrow transplantation is a treatment used for hematologic and non-hematologic disorders. A theory suggests that proliferation of cells in non-body condition helps to increase the efficiency of bone marrow transplant. There are different ways for proliferation of stem cells, in which, most studies have focused on stem cell culture in body-like conditions. The use of amniotic fluid as a rich resource of growth factors is developing in repair of tissues cornea. With regards to this condition, we discuss about the influence of amniotic fluid in proliferation and implantation of blood stem cells. The aim of this study was investigation of human amnion fluid (HAF) in support of growth and proliferation of umbilical cord in order to transpla

    The Effect of Follicular Fluid on the Proliferation and Osteoblastic Differentiation of Human Bone Marrow Mesenchymal Stem Cells

    Atefeh Soltani, Saeid Abroun, Mojtaba Rezazadeh Valojerdi, Bahareh Vahidianfar, Elahe Sadat Hosseini
    Journal PapersInternational Journal of Medical Laboratory , 2019 September 2, {Pages }

    Abstract

    Background and Aims: Bone marrow-derived mesenchymal stem cells (BM-MSCs) are a well-known source of multipotent adult stem cells. Despite using different methodologies of MSCs preparing for clinical applications, the top safest procedure to manipulate these cells, has not yet been determined. Recently, ex-vivo expansion of MSCs for their subsequent implantation, using some biological product, is suggested instead of fetal bovine serum (FBS). Previous studies have shown the effect of follicular fluid (FF)(a dynamic fluid in ovarian follicle) as an additive component in cell culture. Hence, this study aimed to decipher its role on the human BM-MSC proliferation.Materials and Methods: In this study, BM-MSCs at 3 rd passage were cultivated in

    Potential of Polycaprolactone Nanofi-ber Scaffold for Ex Vivo Expansion of Cord Blood-Derived CD34+ Hematopoietic Stem Cells

    SH Mousavi, S Abroun, M Soleimani, SJ Mowla
    Journal PapersInt J Stem Cell Res Ther , Volume 5 , 2019 January , {Pages 059 }

    Abstract

    Background: An efficient and practical ex vivo expansion of cord blood hematopoietic stem cells as an alternative source of HSC transplantation is crucial in understanding the potential of HSC transplantation in treating or supportive therapy in a variety of hematologic and non-hematologic disorders. The aim of this study was an ex vivo expansion of cord blood hematopoietic stem cells in a novel threedimensional polycaprolactone nanofiber scaffold coated with collagen.Methods: After 10-day cultured of cord blood CD34+ cells in 2-Dimensional and 3-Dimensional culture system, the evaluation was performed by qRT-PCR, flow cytometry and clonogenicity.Results: 3-Dimensional Polycaprolactone nano-scaffold coated with collagen provided higher tota

    Immunophenotypic and Immunotypic Evalua-tions of Human Myeloma Cell Lines KMM-1, JJN3, LP1, L363, KMS-12BM and RPMI-8226 for Cell-Line Authentication

    Saeid Abroun
    Journal PapersISMJ , Volume 22 , Issue 5, 2019 December 10, {Pages 278-295 }

    Abstract

    Background: The disease-associated cell lines used in the majority of preclinical trials should be first authenticated and identified to avoid directing research expenditure on wrong cells and obtaining irrelevant results. The present study evaluated the immunophenotypic and immunotypic authenticity of six human myeloma cell lines (HMCLs).Materials and Methods: Cytospin smear and Wright staining were used for invetigating cell morphology, the flowcytometry of the markers CD45, CD2, CD19, CD38 and CD138 for immunophenotypic investigations, capillary electrophoresis for the immunoglobulin secretion potency and immunofixation for the immunotypical investigation of the cells.Results: Given the definite plasma cell morphology, the identity of al

    Umbilical cord blood quality and quantity: Collection up to transplantation

    Seyed Hadi Mousavi, Morteza Zarrabi, Saeid Abroun, Mona Ahmadipanah, Bahareh Abbaspanah
    Journal PapersAsian Journal of Transfusion Science , Volume 13 , Issue 2, 2019 July , {Pages 79 }

    Abstract

    Umbilical cord blood (UCB) is an attractive source of hematopoietic stem cells for transplantation in some blood disorders. One of the major factors that influence on transplantation fate is cord blood (CB) cell count, in addition to human leukocyte antigen similarity and CD34+ cell number. Here, we review the factors that could effect on quality and quantity of CBUs. Relevant English-language literatures were searched and retrieved from PubMed using the terms: CB, quality, collection, and transplantation. The numbers of total nucleated cells (TNCs) and CD34+ cells are good indicators of CB quality because they have been associated with engraftment; thereby, whatever the TNCs in a CB unit (CBU) are higher, more likely they led to successful

    The effect of human amniotic fluid on the survival and proliferation of human myeloma cell lines RPMI8226 and U266 in comparison with the fetal bovine serum

    Fatemeh Mojtahedi, Reza Mansouri, Saeed Abroun
    Journal PapersRazi Journal of Medical Sciences , Volume 26 , Issue 4, 2019 July 1, {Pages 54-69 }

    Abstract

    Background: Multiple Myeloma is a Plasma Cell Malignancy. Since the study of pathogenicity mechanisms and messenger pathways involved in the causative agent cells is important in the laboratory environment and close to the physiological environment of the body, therefore, the best environment for the study of cells in the laboratory environment, an environment most closely resembling the physiological environment of the body. However, the use of fetal bovine serum (FBS) as a supplement to the growth of cells in the cell culture process is very common. Therefore, the aim of this study was to investigate the effect of amniotic fluid on proliferation (Ki-67 and Cyclin-D) and survival (Bax and Bcl2) of both RPMI8226 and U266 Myeloma in comparis

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    دروس نیمسال جاری

    • دكتري
      بيماري هاي عفوني قابل انتقال از طريق تزريق خون و فرآورده هاي خوني ( واحد)
      دانشکده علوم پزشکی، گروه خون شناسي
    • دكتري
      پيشرفت هاي نوين در خون شناسي ( واحد)
    • دكتري
      اصول پيام رساني سلولي ( واحد)
    • دكتري
      اصول پيام رساني سلولي ( واحد)
    • دكتري
      جنين شناسي ملكولي ( واحد)
    • كارشناسي ارشد
      اصول و مباني آزمايشهاي مولكولي خونشناسي ( واحد)
    • كارشناسي ارشد
      خون شناسي 2(بيماريهاي مرتبط با گلبولهاي سفيد) ( واحد)
    • كارشناسي ارشد
      خون شناسي 2(بيماريهاي مرتبط با گلبولهاي سفيد) ( واحد)
    • كارشناسي ارشد
      طب انتقال خون ( واحد)
    • كارشناسي ارشد
      كنترل كيفي در خون شناسي آزمايشگاهي و بانك خون ( واحد)

    دروس نیمسال قبل

    • كارشناسي ارشد
      بيولوژي سلولي و ملكولي پايه ( واحد)
      دانشکده علوم پزشکی، گروه خون شناسي
    • دكتري
      رويكرد تشخيصي بيماري هاي خوني ( واحد)
    • دكتري
      رويكرد تشخيصي بيماري هاي خوني ( واحد)
    • كارشناسي ارشد
      ايمنوهماتولوژي ( واحد)
    • كارشناسي ارشد
      ايمنوهماتولوژي ( واحد)
    • كارشناسي ارشد
      خون شناسي 1( كليات و بيماريهاي مرتبط با گلبولهاي قرمز ) ( واحد)
    • كارشناسي ارشد
      خون شناسي 1( كليات و بيماريهاي مرتبط با گلبولهاي قرمز ) ( واحد)
    • كارشناسي ارشد
      خون شناسي 3 ( هموستاز پلاكتها و بيماريهاي مرتبط با سيستم هموستاز و انعقاد) ( واحد)
    • كارشناسي ارشد
      خون شناسي 3 ( هموستاز پلاكتها و بيماريهاي مرتبط با سيستم هموستاز و انعقاد) ( واحد)
    • كارشناسي ارشد
      تفسير آزمايشهاي خون شناسي ( واحد)
    • دكتري
      بانك هاي سلولي، بافتي و اعضا ( واحد)
    • دكتري
      بانك هاي سلولي، بافتي و اعضا ( واحد)
    • 1396
      ازادپور, شيما
      بررسي امكان پيش بيني وقوع بيماري پيوند عليه ميزبان مزمن(cGVHD) در كودكان پس از پيوند آلوژن سلول هاي بنيادي خونساز با مطالعه پروفايل بيان ژن سلول هاي ايمني زيرگروه هاي T و NKcell
    • 1398
      طاري, كاوه
    • 1398
      قاسمي مهر, نرگس
    • 1399
      جديدطواف, مريم
    • عضویت در شورای پژوهشی دانشکده علوم پزشکی
    • عضو هیات تحریریه Nova J. of Medical and Biological Sciences
    • عضویت در هیات تحریریه نشریه علمی- پژوهشی J of Hematology and Blood Disorders
    • مدیر گروه آموزشی خون شناسی
    • عضو هیوت تحریر مجله cell Journal
    • مدیر فنی بانک خون بند ناف رویان
    • مسئول فنی آزمایشگاه تشخیص طبی
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